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You are investigating a gene with two large introns

Question : You are investigating a gene with two large introns : 2134126

92) You are investigating a gene with two large introns (intron 1 and 2) and three short exons (exons A, B, C). Show the results of R-looping experiments performed with:

(a) mRNA with exons A, B, and C, and single-stranded DNA

(b) pre-mRNA and single-stranded DNA

(c) mRNA with exons A and C only, and single-stranded DNA

93) Sequence analysis of a novel cloned gene reveals that the exons are poorly conserved and the introns are highly conserved. What features would you look for in the RNA that might help in determining possible function of the intronic RNA?

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