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What would be the effect on the function of eIF2 of inhibiting

Question : What would be the effect on the function of eIF2 of inhibiting : 2134193

53) What would be the effect on the function of eIF2 of inhibiting its guanine exchange factor?

Would the outcome be different if eIF2 were phosphorylated?

54) You are investigating a tRNA whose charging specificity appears to be affected by a U11:G24 to U11:A24 base pair in the D stem. Design an experiment using an in vitro reaction to show that changing this base-pair changes the charging specificity of the tRNA.

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