QUESTION 17 You want to amplify a region of yeast : 345299
QUESTION 17 You want to amplify a region of yeast DNA using PCR so that this fragment can be cloned into a plasmid. Which of the following enzymes is needed for the PCR?
A)T4 RNA polymerase
QUESTION 18 Next generation sequencing is much more efficient than the Sanger method because:
A)it uses an RNA template instead of DNA.
B)it uses PCR amplification.
C)it uses gel electrophoresis to resolve end-labeled strands of DNA, and it uses PCR amplification.
D)it uses gel electrophoresis to resolve end-labeled strands of DNA.
QUESTION 19 A specific nucleic acid fragment is used in all of the following techniques, EXCEPT:
B) Northern blotting
C)in situ hybridization
QUESTION 20 Northern blotting is a technique used to detect which one of the following in cells and tissues?