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QUESTION 17 You want to amplify a region of yeast

Question : QUESTION 17 You want to amplify a region of yeast : 345299

QUESTION 17 You want to amplify a region of yeast DNA using PCR so that this fragment can be cloned into a plasmid. Which of the following enzymes is needed for the PCR?

A)T4 RNA polymerase

B)DNA helicase

C)DNA ligase

D)DNA polymerase

QUESTION 18 Next generation sequencing is much more efficient than the Sanger method because:

A)it uses an RNA template instead of DNA.

B)it uses PCR amplification.

C)it uses gel electrophoresis to resolve end-labeled strands of DNA, and it uses PCR amplification.

D)it uses gel electrophoresis to resolve end-labeled strands of DNA.

QUESTION 19 A specific nucleic acid fragment is used in all of the following techniques, EXCEPT:

A)Western blotting

B) Northern blotting

C)in situ hybridization

D)Southern blotting

QUESTION 20 Northern blotting is a technique used to detect which one of the following in cells and tissues?

A) DNA.

B)protein.

C) RNA.

D) carbohydrate.

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