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Hi,

Im doing a biochemistry experiment where we convert newspaper into ethanol by acid hydrolysis, fermentation, and distilation. The acid hydrolysis beaks down the cellulose of the newspaper and isolates glucose molecules from the cellulose.

Some of the resultant glucose is analyzes using thin layer chromatography where a silica gel plate is used as the stationary phase and 7:2:1 n-propanol:ethyl acetate:water is used as the mobile phase.

Visualization is achieved by dipping in a KMnO4 solution. Yellow spots from the glucose are visible on the purple background of the placte after the TLC is done.

The result of the TLC experiment with my glucose isolate is compared to a glucose standard.

My question is, how do i interpret these results. Whar exactly am I comparing and what does this mean. Do I measure how far my glucose isolate has travelled compared to the glucose standard? Or am I analyzing the purity of my sample compared to the glucose standard?

thanks for your help.

 

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Classical Studies 6 Months Ago 5 Views
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