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Hi, I really need up fiuring out how to do this. I'm doing a 1:10 ratio dilution. I know it's regular chem. lab but I can't remember. Equation and help please. Need by tonight.

Use 0.2 ml PCR tubes for these reactions. The thin wall facilitates heat transfer. Be careful to not crush the tubes between your fingers. There is not much writing surface on these tubes so it is best to create a log in your notebook and then number the tubes accordingly.

Reagent

Stock Conc

Final Conc

Dilution

Volume per Sample

Volume per Master Mix (N+1)

Template DNA

5 ul

5X GoTaq Flexi Buf

5X

1X

dNTP Mixture

10 mM

0.2 mM

MgCl2

25 mM

2.5 mM

D1S80 Fwd Primer

10 uM

0.5 uM

D1S80 Reverse Primer

10 uM

0.5 uM

GoTaq Hot Start Polymerase

5U/ul

1.25U

MGW (Molecular Grade Water)

Reagent

Stock Conc

Final Conc

Dilution

Volume per Sample

Volume per Master Mix (N+1)

Template DNA

 

 

 

5 ul

 

5X GoTaq Flexi Buf

5X

1X

 

 

 

dNTP Mixture

10 mM

0.2 mM

 

 

 

MgCl2

25 mM

2.5 mM

 

 

 

D1S80 Fwd Primer

10 uM

0.5 uM

 

 

 

D1S80 Reverse Primer

10 uM

0.5 uM

 

 

 

GoTaq Hot Start Polymerase

5U/ul

1.25U

 

 

 

MGW (Molecular Grade Water)

 

 

 

 

 

 

 

Solution
5 (1 Ratings )

Solved
Classical Studies 10 Months Ago 5 Views
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