x
Info
x
Warning
x
Danger
 / 
 / 
 / 
Consider a cotransfection assay with expression vectors for two

Question : Consider a cotransfection assay with expression vectors for two : 2133871

59) Consider a cotransfection assay with expression vectors for two putative transcription factors that you suspect work together to activate transcription, and with a regulatory region of interest attached to a CAT reporter gene. You also test two deletion () mutants of the regulatory region.

(a) How could you confirm that the transcription factors are transcribed and translated in the transfected cells?

(b) Interpret the results of the CAT assay below. The plus and minus symbols indicate that presence or absence of expression vectors for the listed components in the transfection assay.

(c) How would you demonstrate protein-protein interaction between the two transcription factors in vivo and in vitro? Show sample positive results.

(d) Describe protocols to further characterize direct binding of the proteins to DNA in vitro and in vivo. Show sample results.

Solution
5 (1 Ratings )

Solved
Biology 1 Week Ago 34 Views
This Question has Been Answered!

Related Answers
Unlimited Access Free
Explore More than 2 Million+
  • Textbook Solutions
  • Flashcards
  • Homework Answers
  • Documents
Signup for Instant Access!
Ask an Expert
Our Experts can answer your tough homework and study questions
180472 Biology Questions Answered!
Post a Question