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65) As they light up their cigarette, a friend of yours tells you that

Question : 65) As they light up their cigarette, a friend of yours tells you that : 2134366

65) As they light up their cigarette, a friend of yours tells you that they have heard that benzo(a)pyrene in cigarette smoke is not really a carcinogen. Set them straight.

66) You are designing a somatic cell gene therapy protocol for spinal muscular atrophy, a genetic disorder that is caused by a defect in the Survival of Motor Neurons (SMN) gene. You have cloned the SMN cDNA.

(a) What is the minimal cis-acting DNA regulatory element you would need to ligate to the SMN cDNA to get transcription of the cDNA in cells?

(b) Assume that you decide to use retroviral-mediated gene transfer to introduce the SMN cDNA into the patient's target cells ex vivo. What are the advantages and disadvantages of this choice of vector for gene delivery? Is there another vector that would work better?

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